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Gabriele Mues

MuesAssistant Professor
Department of Biomedical Sciences

3302 Gaston Ave.
Room 448
Dallas, Texas 75246
Phone: 214-828-8291
Fax: 214-874-4538

Education and Post-Graduate Training

M.D. (USA), USMLE 1, 2 (1997) and 3 (2003)

Dipl. Psych., University of Bochum, Germany (1978)

Dr. med., University of Essen, Germany (1975)

M.D. (Germany), University of Essen, Germany (1973)

Cand. Med., University of Marburg, Germany (1970)

Career History

Assistant Professor, Department of Biomedical Sciences, Texas A&M University Baylor College of Dentistry (2008-present)

Research Associate I, Department of Biomedical Sciences, Texas A&M University Baylor College of Dentistry (2006-2008)

Research Fellow, Department of Pathology, University of Texas Southwestern Medical Center, Dallas (2001-2002)

Fellow, Molecular Diagnostics, University of Texas Southwestern Medical Center, Dallas (2001-2003)

Research Fellow, Department of Human Genetics, University of Texas Southwestern Medical Center, Dallas (1999-2000)

House Staff, Parkland Memorial Hospital, Dallas (1998-2003)

Resident, Clinical Pathology, University of Texas Southwestern Medical Center, Dallas (1998-2001)

Graduate Faculty, Baylor University, Waco, TX (1995-1997)

Adjunct Associate Professor, Texas A&M University Baylor College of Dentistry, Dallas (1996-1997)

Director, M. Crowley Research Lab, Baylor University Medical Center, Dallas (1994-1997)

Associate Graduate Faculty, Baylor University, Waco, TX (1992-1995)

Associate Director, M. Crowley Research Lab, Baylor University Medical Center, Dallas (1992-1993)

Assistant Instructor, Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas (1989-1991)

Assistant Instructor, Department of Microbiology, University of Texas Southwestern Medical Center, Dallas (1988-1989)

Research Associate, Department of Psychiatry, University of Texas Southwestern Medical Center, Dallas, TX (1982-1987)

Research Fellow, School of Public Health, University of California, Berkeley (1981-1982)

Research Fellow, Department of Psychology, Univeristy of Bochum, Germany (1978-1980)

Intern, Internal Medicine, Elisabeth Krankenhaus, Bochum, Germany (1974-1975)

Intern, Surgery, Staedtische Krankenanstalten Karlsruhe, Germany (1974)

Research Interests

Most of my projects involve genetic research with human samples in order to study congenital dental/craniofacial problems.  We are continuing the recruitment of new patients with non-syndromic tooth agenesis for our tooth agenesis study.  A candidate gene approach is used to detect new mutations in known tooth agenesis genes and new causative genes in these patients.  The list of candidate genes for this study includes PAX9, MSX1, AXIN2, DLX1, DLX2, LEF1, GLI2, GLI3, PITX2, LHX6, BARX1, MSX2, ACTVIN-beta A, and EDA1.  Drs. Chalothorn and Beeman in Kentucky found a higher prevalence of mild tooth agenesis in ovarian cancer/cyst patients and asked us to do an ovarian cancer/tooth agenesis association study with their samples.  We investigated buccal swab samples from these patients for mutations/polymorphisms in the major known tooth agenesis genes PAX9, MSX1, AXIN2 and in the homeo-domain proteins BARX1 and BARX2, both of which play a role in the development of craniofacial structures as well as tumors.  We have found that a common polymorphism in MSX1 is found more frequently in affected individuals and that there are several new polymorphisms in the BARX genes; some must be investigated for pathogenic potential.  We have also found one mutation in the AXIN2 gene that could contribute to ovarian cancer development and one EDA mutation that probably causes the tooth agenesis in the affected patient.

The clinical objectives (a and b below) of our EDA-gene mutations in non-syndromic tooth agenesis project are to provide the dental practitioner with guidelines for the referral of tooth agenesis patients to geneticists because EDA-associated selective tooth agenesis may be curable in the near future by post-natal injection of recombinant EDA protein.  For this project, we plan the:

a. Determination of the prevalence of EDA-gene mutations in selective tooth agenesis;

b. Dental phenotype characterization of EDA-associated selective tooth agenesis;

c. Biochemical characterization of the molecular malfunction of mutated EDA protaions (in collaboration with Dr. Pascal Schneider, Lausanne, Switzerland).

Two studies are being conducted in collaboration with Dr. Hitesh Kapadia: 1) Pierre Robin Study.  We have collected buccal swab samples from 33 patients with the Pierre Robin syndrome.  The HAND1 and HAND2 genes, as well as the branchial arch enhancer elements in in the HAND2 gene, are screened for mutations.  Twenty-nine samples have been analyzed to date (some only partially), and one definitive mutation in HAND2 has been identified; 2) Functional Analysis of the PAX9 Gene. This project investigates the capability of wild-type and mutant PAX9 proteins to bind certain promoters and activate the transcription of target genes.  Protein-protein interactions between PAX9 and other homeo box partner genes such as MSX1 and 2 are also studied in order to increase our understanding of tooth development.

Research Experience

Development of clinical molecular tests

FV Leiden and Prothrombin mutations by TaqMan assay (2002-2003)

Immunoglobulin/T-cell receptor clonality assessment by fluorescent capillary electrophoresis (2002-2003)

Tumor LOH assessment by fluorescent capillary electrophoresis (2002-2003)

Bone marrow graft by STR analysis (2002-2003)

Assessment of clinical significance

SNP analysis in the tissue factor pathway inhibitor gene (2001-2002)

SNP analysis in lipid metabolism genes: Verification of mutations in Affymetrix variant detector arrays (1999-2000)

K-ras, p53 mutations, ras and erb-B2 expression in NSCLC and colon cancer (1994-1997)

Basic research

Detection of human tooth agenesis genes (2006-present)

Detection of radiation-induced gene products by the Differential Display method (1993-1996)

Assessment of the heat shock response to x-irradiation (1991-1992)

Characterization of the structure and function of the human choline acetyl transferase gene promoter (1989-1991)

Construction of enzyme-labelled hybridization probes, M13-based universal hybridization probes (1988-1989)

Isolation and characterization of a human hsc70 gene family (1982-1987)

Development of bioassays for neuropeptides (1981-1982)

Behavioral effects of experimental amygdale lesions (Master's thesis in Psychology) (1777-1979)

Urinary enzyme excretion in hypertension and renal disease (Doctoral thesis in Medicine) (1972-1974)

Recent Grants

Signaling mechanisms in early tooth development; NIH/NIDCR R01 DE01947-01; 2008-2010, $345,781 (Co-Investigator).  American Recovery and Reinvestment Act administrative supplement; 2009-2010;  $185,638.

EDA pathway mutations in non-syndromic tooth agenesis; NIH/NIDCR R03 DE0196554-01A2; 2010-2012 (PI).

Selected Publications

Huang Y, Lu Y, Mues G, Wang S, Bonds J and D’Souza RN (2013). Functional evaluation of a novel tooth agenesis-associated BMP4 prodomain mutation (2013).  Eur J Oral Sci 121:313-318.

Siyam A, Wang S, Qin C, Mues G, Stevens R, D'Souza RN, Lu Y (2012).  Nuclear localization of DMP1 proteins suggests a role in intracellular signaling.  Biochem Biophys Res Commun 424:641-646.

Kong H, Wang Y, Patel M, Mues G, D'Souza RN (2011).  Regulation of Bmp4 expression in odontogenic mesenchyme: From simple to complex.  Cells Tissues Organs 194:156-160.

Wang Y, Kapadia H, Long H, Mues G, D'Souza RN (2011).  MSX1 mutations: How do they cause tooth agenesis?  J Dent Res 90:311-316.

Mues G, Tardivel A, Willen L, Kapadia H, Seaman R, Frazier-Bowers S, Schneider P, D'Souza RN (2010).  Functional analysis of Ectodysplasin-A mutations causing selective tooth agenesis.  Eur J Hum Genet 18:19-25.

Maciejewska I, Qin D, Huang B, Sun Y, Mues G, Svoboda K, Bonewald L, Butler WT, Feng JQ, Qin C (2009).  Distinct compartmentalization of dentin matrix protein 1 fragments in mineralized tissues and cells.  Cells Tissues Organs189:186-191.

Mues G, Griggs R, Hartung A, Whelan G, Best L, Srivastava A, D'Souza R (2009).  From ectodermal dysplasia to selective tooth agenesis.  Am J Med Genet A 149A:2037-41.

Wang Y, Groppe JC, Wu J, Ogawa T, Mues G, D'Souza RN, Kapadia H (2009).  Pathogenic mechanisms of tooth agenesis linked to paired domain mutations in human PAX9.  Hum Mol Genet 15:2863-74.

Wang Y, Wu H, Wu J, Zhao H, Zhang X, Mues G, D'Souza RN, Feng J, Kapadia H (2008).  Identification and functional analysis of two novel PAX9 mutations.  Cells Tissues Organs 189:192-197.

Kapadia H, Mues G, D'Souza R (2007).  Genes affecting tooth morphogenesis. Orthod Craniofac Res 10(4):237-244.

Cheng Y-Sl, Mues G, Wood D, Ding J (2006).  Aromatase expression in normal human oral keratinocytes and oral squamous cell carcinoma.  Arch Oral Biol 51:612-620.

Mues GI, Sarode R (2002). Allele frequencies of tissue factor pathway inhibitor polymorphisms in African-American, Hispanic and Caucasian populations. Thromb Haemost 88:875-877.

Mues G, Wians FH Jr, Kroft SH (2001).  EDTA-induced pseudo-gray platelet syndrome.  Lab Med 32:361-364.

Garcia CK, Mues G, Liao Y, Hyatt T, Patil N, Cohen JC, Hobbs HH (2001).  Sequence diversity on genes of lipid metabolism.  Genome Res 11:1043-52.

Nizzi FA Jr, Mues G (2002).  Hemorrhagic problems in obstetrics, exclusive of disseminated intravascular coagulation.  Hematol Oncol Clin North Am 14:1171-82.  Review.

Tong AW, Su D, Mues G, Tillery GW, Goldstein R, Klintmalm G, Stone MJ (1996).  Chemosensitization of human hepatocellular carcinoma cells with cyclosporine A in post-liver transplant patient plasma.  Clin Cancer Res 2:531-539.

Nemunaitis J, Klemow S, Tong A, Courtney A, Johnston W, Mack  M, Taylor W, Solano M, Stone M, Mallams J, Mues G (1998).  Prognostic value of K-ras mutation, ras oncoprotein and c-erb B-2 oncoprotain expression in adenocarcinoma of the lung.  Am J Clin Oncol 21:155-160.

Nemunaitis J, Cox J, Meyer W, Courtney A, Mues G (1997).  Irinotecan hydrochloride (CPT-11) resistance identified by K-ras mutation in patients with progressive colon cancer after treatment with 5-fluorouracil (5-FU).  Am J Clin Oncol 20:527-529.

Chang YT, Hyland K, Mues G, Marsh JL (1997).  Human hair follicles as a peripheral source of tyrosine hydroxylase and aromatic L-amino acid decarboxylase mRNA.  Neurosci Lett 222:210-212.

Tong A, Zhang YA, Nemunaitis J, Mues G (1997).  K-ras ribozyme for lung cancer.  In : Scanlon F, ed: Therapeutic Applications of Ribozymes.  Methods in Molecular Medicine.

Chang YT, Mues G, Hyland K (1996).  Alternative splicing in the coding region of human aromatic L-amino acid decarboxylase mRNA.  Neurosci Lett 202:157-160.

Chang YT, Mues G, Pittelkow MR, Hyland K (1996).  Cultured human keratinocytes as a peripheral source of mRNA for tyrosine hydroxylase and aromatic L-amino acid decarboxylase.  J Inherit Metab Dis 19:239-242.

Tong AW, Zhang BQ, Mues G, Solano M, Hanson T, Stone MJ (1994).  Anti-CD40 antibody binding modulates human multiple myeloma clonogenecitiy in vitro.  Blood 84:3026-33.

Hersh LB, Kong CF, Sampson C, Mues G, Li YP, Fisher A, Hilt D, Baetge EE (1993).  Comparison of the promoter region of the human and porcine choline acetyltransferase genes: Localization of an important enhancer region.  J Neurochem 61:306-314.

Munn TZ, Mues GI (1988).  Highly conserved repeats in heat-shock introns.  Nature 332:789 (Correspondence).

Munn TZ, Mues GI (1986).  Human lipocortin similar to ras gene products.  Nature 322:314-315 (Correspondence).

Mues GI, Munn TZ, Rase JD (1986).  A human gene family with sequence homology to Drosophila melanogaster Hsp70 heat shock genes.  J Biol Chem 261:874-877.

Mues G, Fuchs I, Wei ET, Weber E, Evans CJ, Barchas JD, Chang JK (1982).  Blood pressure elevation in rats by peripheral administration of Tyr-Gly-Gly-Phe-Met-Arg-Phe and the invertebrate neuropeptide Phe-Met-Arg-Phe-NH2. Life Sci 31:2555-61.

Maruhn D, Fuchs I, Mues G, Bock KD (1976).  Normal limits of urinary excretion of eleven enzymes.  Clin Chem22:1567-74.

Gilow L, Maruhn D, Fuchs I, Mues G, Strozyk K, Bock KD (1974).  Urine lactate dehydrogenase, N-acetyl-beta-D-glucosaminidase and trehalase excretion in primary hypertension and various kidney diseases.  Verh Dtsch Ges Inn Med 80:291-295.

Specialty Training/Board Certifications

Board certification in Clinical Pathology (2003)

National Service/Recognition

Medical licensing examination, Germany: Summa cum laude (1974)

Award for outstanding thesis in Medicine, Germany (1985)

Research fellowship from "Deutsche Forschungsgemeinschaft" (1981-1983)

Last edited by: werchan 11/18/2014

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